COLUMN DIMENSIONS {I.D. x L(mm)} VOID VOLUME {ml}
1.0 x 100 0.06 1.0 x 150 0.08 1.0 x 250 0.14 1.0 x 300 0.17
2.1 x 100 0.24 2.1 x 150 0.37 2.1 x 250 0.61 2.1 x 300 0.73
4.6 x 100 1.16 4.6 x 150 1.75 4.6 x 250 2.90 4.6 x 300 3.49
7.8 x 100 3.35 7.8 x 150 5.02 7.8 x 250 8.36 7.8 x 300 10.04
10.0 x 100 5.50 10.0 x 150 8.25 10.0 x 250 13.75 10.0 x 300 16.49
21.0 x 100 24.25 21.0 x 150 36.37 21.0 x 250 60.61 21.0 x 300 72.74
50.0 x 100 137.45 50.0 x 150 206.17 50.0 x 250 343.61 50.0 x 300 412.33
Void Volume (ul) = (d^2 *Pi * L * Pore Volume) / 4
(Note: Column Diameter and Length in mm)
* Notes : Minimize all extra-column volume when testing. Assumes an Average Pore Volume of 0.70 (70%) on a bare, fully porous spherical silica support.
Superficially porous particles (e.g. Fused-Core, Core-Shell) have less volume than the standard fully porous supports (e.g. 50%).Depending on any endcapping chemistries used (e.g. C8 - C18) the calculated value can differ from the measured value.Always inject an unretained compound onto your specific column to determine the actual column void volume.For many common RP columns run with at least 20% organic mobile phase content, Uracil or Thiourea may be tried, but some inorganic salts(e.g. sodium nitrite and sodium nitrate) have also been shown to work as well.For many normal phase silica, cellulose, amylose or brush-type normal phase supports, 1,3,5-Tri-t-butyl benzene (TTBB) has been used with some success.
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