From time to time we will add various 'hints and tips' to this web page which we feel are of interest to anyone performing HPLC, UHPLC, LC-MS  or SFC analysis. Many of these tips are taught in our specialized training courses. Often, they are the result of problems we see people experiencing in the field. As scientific educators and consultants, we try to pass on any practical information that may help others improve the way in which they conduct their research.

To see even more HPLC Hints and Tips (for free), please follow our professional chromatography blog dedicated to helping others learn more about this analytical technique; Link:  https://hplctips.blogspot.com/

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·          TABLE OF CONTENTS:

 

TIP #	DESCRIPTION AND WEB PAGE LINK (click to view)	DATE ADDED
101	Inline HPLC Degassing Systems / Modules	05/29/2010
102	Reference Wavelengths (as used in HPLC UV/VIS Detectors)	05/30/2010
103	Flow Cell Volume & Path Length (Method Transfer Issues)	06/12/2010
104	Chiral HPLC / SFC Method Development (Alcohol's)	07/07/2010
105	Power & Surge Protection for Computers & Analytical Instruments	07/18/2010
106	Determination of HPLC Column Dead Time / Dwell Volume, (T0)	08/01/2010
107	Chiral HPLC and SFC Column Screening Strategies	09/20/2010
108	HPLC TO UHPLC Conversion Notes	11/22/2010
109	UV VIS Detector Signal Bandwidth	12/06/2010
110	HPLC Pump Solvent Compressibility Values	01/16/2011
111	Proteomics and LC-MS, MS/MS Software Tools	04/21/2011
112	Determining the Data Acquisition Rate For Your HPLC Detector	05/30/2011
113	How Do C18 Phases Differ?	07/18/2011
114	Calculate Pressure Drop Across an HPLC / UHPLC Column	08/17/2011
115	Adduct Formation In LC-MS Analysis (Esp. ESI)	11/17/2011
116	Hydrophilic Interaction Chromatography (HILIC)	02/21/2012
117	Techniques To Enhance Negative Mode ESI in LC/MS	04/17/2012
118	Optional Seal Wash & Flushing the HPLC System	08/24/2012
119	Common HPLC UHPLC Calculations	01/01/2013
120	Adjusting Mobile Phase Flow Rate to Maintain HPLC / UHPLC Linear Velocity When Column Diameter Is Changed	04/01/2013

 To see more free HPLC Hints and Tips, please subscribe to our blog; https://hplctips.blogspot.com/

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Chiralizer™ Services, L.L.C.  USA

© Copyright 2010 - 2015, Chiralizer™ Services, L.L.C. All Rights Reserved (Worldwide). Chiralizer, Column Swapper & LC Spiderling are trademarks of Chiralizer Services, LLC  USA. *These "Hints & Tips" are protected by copyright and are not to be copied or duplicated in whole or part without the written permission of Chiralizer Services, LLC.